FastQCFastQC Report
Sun 14 Apr 2019
SRR6576730_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576730_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387627
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8250.21283347135261474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.14111504100591546No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5160.13311766208236267No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4870.1256362430893617No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4410.11376916468667045No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4320.11144734499918736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9800.054.3043981
TATAACG405.6692347E-447.0047072
ATCAACG13400.039.63833
TCAACGC13800.038.489364
CAACGCA13700.037.741015
AACGCAG13600.037.6728946
GTACATG23400.036.6301041
ACATGGG24000.035.4493833
TACATGG24200.034.962182
CATGGGT6050.033.4083064
TATCAAC16150.033.1797942
TACACCG600.004155572531.3364725
ACGCAGA16650.030.7678837
CTATTAA1701.1823431E-1030.4737871
ATGGGTA4350.030.2559055
CGCAGAG17000.029.8579648
GGTACCT3500.029.5420078
GGGTACC3350.029.4618387
ATGGGAG5800.028.3649085
CATGGGA12600.028.3520494
Produced by FastQC (version 0.11.2)