FastQCFastQC Report
Sun 14 Apr 2019
SRR6576731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355988
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7910.2221985010730699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.14747688124318797No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5000.14045417261256No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4600.12921783880355517No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4490.12612784700607885No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3810.1070260795307707No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3780.10618335449509533No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3620.10168882097149343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9850.052.0174641
CCGCTAA405.679268E-446.9867974
GTACTAG652.7767946E-643.3907131
CGCTAAA450.00101441341.7660455
AACGCAG12350.040.3287476
ATCAACG12500.040.22073
CAACGCA12650.039.743775
TCAACGC12750.039.4320564
ACATGGG21950.039.1735043
GTACATG22250.038.661611
TACATGG22650.037.9788482
CCCGCTA500.001702798137.5894363
CATGGGG7700.035.392654
GCAAACG801.155292E-535.2549551
CGCAGAG14350.035.035458
ACGCAGA14250.034.9515847
CATGGGA11250.034.2481584
TATCAAC14850.033.870082
ATGGGAG5050.031.6346765
CAAACGC902.5864472E-531.3377362