FastQCFastQC Report
Sun 14 Apr 2019
SRR6576731_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576731_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355988
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7800.21910850927559355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.15253323145724013No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4910.1379259975055339No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4270.1199478634111262No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3930.11039697967347215No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3650.10253154600716877No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3620.10168882097149343No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3560.1000033709001427No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3560.1000033709001427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT205.679437E-446.99297778-79
GTACATG21400.038.0698661
GTATCAA9450.037.8730931
TACATGG21500.037.594382
ACATGGG22000.036.9535683
GTATTAT1204.110916E-935.3191531
CATGGGA10850.033.3498544
ATAAGAC851.7531876E-533.1715133
CGGCTTA300.004162970531.32865318-19
AACGCAG12200.030.0446916
CATGGGG8750.029.5384434
ATAGATC804.7785102E-429.3706113
CTAGTAC804.7785102E-429.3706113
ATGGGAG5550.028.788495
ATCAACG12300.028.2721963
TCAACGC12600.027.972014
CAACGCA12800.027.902085
GGGTACC2900.027.5476077
CATGGGT5550.027.095054
ATGGGTA3500.025.5104735