FastQCFastQC Report
Sun 14 Apr 2019
SRR6576732_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576732_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189154
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5160.272793596751853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.17869037926768666No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3090.1633589561944236No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2620.1385114774205145No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2510.13269611004789747No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2380.12582340315298646No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT2300.12159404506381043No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2240.11842202649692843No Hit
GAGTAAGCCATTCCCTTTAGACTTGGTTGGGCAGCTGTCACATGGCTGAC2160.11419266840775241No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2150.11366399864660541No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2130.11260665912431142No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG2110.1115493196020174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2070.1094346405574294No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2070.1094346405574294No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1960.10361927318481237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6750.057.2010151
TTCGTTA250.005229706456.3988887
ACAATTC250.005229706456.3988888
CTAGACA405.6651805E-446.9990774
AACGCAG8800.044.8627556
ATCAACG8650.044.5540353
TCAACGC8700.044.2979774
CAACGCA8750.044.0448465
ATAACGC450.001011904141.776963
CATGGGG5550.040.6478464
ACGCAGA9800.040.2849247
GTACATG14250.039.981971
TACATGG14350.039.6298832
CGCAGAG10050.039.2828148
ACATGGG14250.038.9185333
TATCAAC10300.037.873042
AATAACG500.001698599437.599262
AGTCGCT500.001698599437.599268
TAACGCC651.4091944E-436.1531334
GCAGAGT11300.034.9373669
Produced by FastQC (version 0.11.2)