FastQCFastQC Report
Sun 14 Apr 2019
SRR6576733_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576733_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170013
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4610.271155735149665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.1970437554775223No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2880.16939881067918336No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2800.16469328816031717No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT2730.16057595595630922No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2520.14822395934428542No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2490.14645938839971062No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2270.13351920147282856No Hit
GAGTAAGCCATTCCCTTTAGACTTGGTTGGGCAGCTGTCACATGGCTGAC2230.13116644021339546No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2200.1294018692688206No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2080.12234358549052132No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2020.11881444360137165No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2010.11822625328651339No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1980.11646168234193857No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG1820.10705063730420616No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1820.10705063730420616No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1710.10058054384076512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG851.4006218E-1049.802731
TGCGACG205.6743727E-446.9944122-23
GTATCAA6350.045.184111
TAGTACT608.798545E-539.162014
TACTAGT500.001696488837.606592
ATGCGAC250.001701335437.5955320-21
CGCACTA250.001701335437.5955358-59
AACGCAG8100.035.9710276
CATGGGT3100.034.866824
ATCAACG8400.034.686353
CATGGGC2050.034.3861544
CAACGCA8500.034.2782755
TCAACGC8500.034.2782754
TACATGG13000.033.990572
ACATGGG12700.033.673163
CGATAAT352.1795339E-433.57731666-67
GTACATG13250.033.3688741
ATGGGTA1606.002665E-1132.308665
CTGCGTG600.00414157331.3480479
TACCCTT600.004153523631.3296075
Produced by FastQC (version 0.11.2)