FastQCFastQC Report
Sun 14 Apr 2019
SRR6576734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672243
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17230.2563061273973846No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9600.142805503367086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.1262936170402667No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8080.12019463200063073No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7540.11216182243623213No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7390.10993048644612141No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7340.10918670778275119No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7040.10472403580252974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16600.048.1551251
GTACATG42650.040.0211261
TACATGG42950.039.522622
ACATGGG42950.039.0643463
ATCAACG20650.038.0077323
TCAACGC21050.037.2854924
CAACGCA21500.037.160885
AACGCAG22200.036.200846
ATGGGTA7050.033.9982535
CATGGGT10850.033.7862784
GGTACCT6500.033.2598278
CATGGGG16450.032.8554534
GGGTACC6900.032.0128447
CGCAGAG25750.031.392568
ACGCAGA26350.030.8560987
CATGGGA20550.030.4169274
TATCAAC26650.029.9953142
ATGGGGG6400.028.6391535
GGTATCA7850.028.153341
GCAGAGT29400.027.335339