FastQCFastQC Report
Sun 14 Apr 2019
SRR6576734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672243
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13480.2005227276446166No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9760.14518559508987078No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9710.14444181642650053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.1254010826442224No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8250.12272347945608954No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7500.11156679950553594No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7480.11126928804018786No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7200.10710412752531452No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6950.1033852342084633No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6750.10041011955498236No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6740.1002613638223083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15500.052.9372861
CAACGCA20650.039.8325585
TCAACGC20650.039.8325584
ATCAACG20650.039.6078873
GTACATG39300.039.237321
AACGCAG21050.038.8494646
TACATGG40100.037.979842
ACATGGG40850.037.05243
CATGGGA21850.033.3426744
ACGCAGA25450.032.130477
TATCAAC26200.031.397082
CGCAGAG26150.031.0906628
ATGGGAG9850.029.585285
ATGGGTA6200.029.5660385
GGTATCA5950.029.3243831
CATGGGG14300.029.2532524
GGGTACC6150.027.5095187
GCAGAGT30100.027.010669
TACTGCG700.00883765326.8545287
CATGGGT11050.026.3723984