FastQCFastQC Report
Sun 14 Apr 2019
SRR6576735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617484
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15700.25425760019692817No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8950.14494302686385396No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8050.13036775041944407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.12988190787129708No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7790.12615711500217008No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7520.12178453206884712No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6980.11303936620220117No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6850.11093404849356421No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6300.10202693511086927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16450.046.5738371
GTACATG38500.038.7007031
TACATGG38200.038.5124632
ACATGGG38800.037.4202273
ATCAACG20650.036.6340943
AACGCAG20700.036.318616
TCAACGC20700.036.091624
CAACGCA20750.036.0046545
GGTATCA7700.032.352321
CATGGGA19400.030.7596684
CATGGGG14850.030.691984
CGCAGAG24500.030.6855228
ACGCAGA24850.030.253337
GTACTAG804.776902E-429.3765181
CATGGGT9450.029.3359264
TATCAAC26300.028.9520662
ATGGGTA6750.027.1481655
ATGGGGA7450.026.4894335
CTAACTC1258.033017E-626.3128363
GCAGAGT29150.025.9517659
Produced by FastQC (version 0.11.2)