FastQCFastQC Report
Sun 14 Apr 2019
SRR6576735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617484
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14530.2353097408191953No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9600.15546961540703888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.13830317870584502No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7770.1258332199700721No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7130.11546857894293616No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6810.1102862584293682No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6780.10980041588122122No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6720.10882873078492722No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6630.10737120314048623No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6600.10688536059233925No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6360.10299862020716326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG250.0052377756.3921785
GTATCAA15400.045.5230141
GTACATG35800.038.90221
TACATGG36050.038.8493542
ATGGGTA5250.037.5947885
ACATGGG37500.036.968213
ATCAACG20200.034.1982273
TCAACGC20350.034.177084
CAACGCA20700.034.053255
AACGCAG21000.034.014336
CATGGGT8600.032.2397164
GGTACCT5000.031.9762828
GTACTAG1052.046052E-631.3670881
GTATAAC753.2531915E-431.3670861
GGGTACC5100.031.3289877
CATGGGA21000.028.8674264
CATGGGG12600.028.7182394
TGGGTAC5750.028.6047256
ACGCAGA25050.028.1398097
CGCAGAG24950.028.0824368