FastQCFastQC Report
Sun 14 Apr 2019
SRR6576736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332864
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4770.14330176889059795No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3950.11866708325322053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7600.050.692211
CATATAG405.6809804E-446.983031
CAACGCA10150.038.4196135
TCAACGC10050.038.3344084
ATCAACG10150.037.9567263
AACGCAG10550.037.8536226
CGTATAA250.00169950937.60901642-43
GGTATCA3200.033.769051
TATCAAC12000.032.105072
TTAAAGC600.00416413131.3220143
GTACATG16950.030.7676451
ATGGGTA2750.030.7525255
ACGCAGA12750.030.5850287
ACATGGG16950.030.2132723
GTAATAC953.7536003E-529.673493
TACATGG17800.029.5623532
CGCAGAG13750.028.3606648
CTCTTAA2400.027.4067631
GGGTACC2750.027.3355777
GTCGCAC350.00882307126.86358642-43
Produced by FastQC (version 0.11.2)