FastQCFastQC Report
Sun 14 Apr 2019
SRR6576736_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576736_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332864
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6150.18476014228033072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.17033983849259757No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4900.147207267833109No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4300.1291818880984426No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3850.1156628532974428No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3710.11145693135935397No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG3550.10665016343010959No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3510.10544847144779851No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT3410.10244424149202078No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3360.1009421265141319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCC156.902735E-493.983323
GTATCAA9350.052.9224361
TCAACGC11500.042.4968074
CAACGCA12000.041.90095
ATAGACG450.001013801241.7703678
ATCAACG11700.041.7703673
CTATACA802.4549627E-741.1177064
AACGCAG12350.040.7134256
GGTATCA3350.039.3890341
TAGACGA500.001701773437.593339
ACGCAGA14100.035.660347
TATCAAC13850.035.2914622
CGCAGAG14500.034.6766058
GTCTTGA550.00267879534.273581
GTACATG16500.033.1311261
ACATGGG16900.032.532693
TACATGG17950.031.419762
CCATATA600.004157326631.3324812
GCAGAGT16100.031.2304849
TACAATC804.778634E-429.369797