FastQCFastQC Report
Sun 14 Apr 2019
SRR6576737_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576737_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386377
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7540.1951461914140852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.1656413295822474No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5410.1400186864124935No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4470.11569011613010091No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4370.11310197035537829No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4040.10456108929879367No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3990.10326701641143235No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3920.10145531436912654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.055.239571
AACGCAG14900.038.171156
ATCAACG14800.038.111473
TCAACGC15100.037.665574
GTACATG25100.037.521491
CAACGCA15100.037.3542825
TACATGG25100.037.266232
ACATGGG25750.036.142993
CATGGGA13200.033.4726454
TATCAAC17150.033.4373472
TATAGAG1309.145879E-932.541335
CATGGGC4350.032.416654
CGCAGAG17950.031.6811438
ACGCAGA18050.031.5056237
ATGGGCG902.5879437E-531.3360965
ATGGGAG6550.028.7048195
GGTATCA4450.027.512951
GCAGAGT20600.027.377519
GTATTAT1406.245282E-726.908271
GTATTAC700.0087473726.908271
Produced by FastQC (version 0.11.2)