FastQCFastQC Report
Sun 14 Apr 2019
SRR6576738_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576738_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354335
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7750.21871957328516797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.20884191513680556No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4740.13377171320925113No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4310.1216363046269773No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4170.11768524136763231No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4150.11712080375915447No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3960.11175864647861486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3940.111194208870137No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3680.10385651995992493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC250.00523697956.389333
GTATCAA11150.047.683841
TACATGG25250.042.2514342
ACATGGG25300.041.976253
GTACATG25650.041.8229941
CATGGGT5900.041.415894
ATCAACG14250.038.5821723
AACGCAG14050.038.4624756
TCAACGC14600.037.0135424
CAACGCA14650.036.887225
CATGGGG8000.035.8307234
GGTACCT2950.035.0689628
GGGTACC3000.034.4601447
ATGGGTA3550.034.4160235
TGGGTAC3150.034.3109666
TCTAGAC702.18439E-433.565083
CGCAGAG16800.032.4691548
CATGGGA14250.032.3166924
ACGCAGA16950.032.1591077
TATCAAC17100.031.881512