FastQCFastQC Report
Sun 14 Apr 2019
SRR6576740_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576740_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229375
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6050.26376021798365124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.18397820163487738No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3620.15782016348773842No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3460.15084468664850137No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3180.1386376021798365No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3130.13645776566757492No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2910.12686648501362396No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2820.12294277929155313No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2670.11640326975476839No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2540.11073569482288828No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2520.10986376021798365No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2400.10463215258855586No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2330.10158038147138965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.045.458631
GTACATG21500.038.5021931
TACATGG21550.038.16132
ACATGGG21650.037.550923
ATGGGAG4000.036.4195065
ATGGGCG651.4112167E-436.1483965
AACGCAG13200.035.6006936
ATCAACG13500.034.461473
GTCTTAC550.002702911934.2064971
CAACGCA14000.033.566375
CATGGGA12200.033.511344
TCAACGC14100.032.995024
ATGGGGG3300.032.7526365
CATGGGG6850.032.2433134
TATCAAC14750.032.17822
ACGCAGA15200.030.2980657
CGCAGAG15350.029.7217678
CATGGGT4150.029.4413454
GTGTAAG650.006122409428.9439561
AGGCGTG650.006148569828.9187157
Produced by FastQC (version 0.11.2)