FastQCFastQC Report
Sun 14 Apr 2019
SRR6576741_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576741_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372726
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8720.23395201837274568No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5040.13521997392186216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.13146386353514378No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4700.1260979915541175No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4510.12100041317214254No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4200.11268331160155182No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4080.10946378841293605No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4060.1089272012148334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9800.046.1425671
GTACATG22350.041.5188521
TACATGG22600.039.926182
ACATGGG22750.038.8366243
ATCAACG12550.036.698423
AACGCAG12600.036.552796
TCAACGC12800.036.3488124
CAACGCA12850.036.207385
CATGGGG9100.033.0524444
ACGCAGA14650.031.4378957
CGCAGAG14800.031.1192688
TATCAAC14500.031.1148872
CATGGGA12450.030.1985194
CATGGGT5950.030.0145364
GGAGTAC650.006151178428.9208898
GTACTAG856.720073E-427.708161
GCAGAGT17150.027.6772089
ATGGGGA5550.027.097055
TATACCC700.00883175726.8551124
ATGGGTG2650.026.601765
Produced by FastQC (version 0.11.2)