FastQCFastQC Report
Sun 14 Apr 2019
SRR6576742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340898
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8800.2581417315443329No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4920.1443246953634225No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4760.1396312093353437No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4750.13933786645858878No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4650.13640443769103955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.12291066536031306No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3630.10648346426203734No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3530.1035500354944881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAG450.001012219241.783911
GTATCAA10100.040.9565051
GTACATG22100.037.222661
TACATGG23250.035.1793562
ACATGGG23050.034.857453
AACGCAG11350.034.7739376
TAATAGA550.00271238734.1868322
ATCAACG12000.033.28193
CAACGCA12050.033.14385
TCAACGC12400.032.2082944
CATGGGG8200.031.515144
GCATTAT600.00415392731.337931
TTCCGAC1052.0644566E-631.324147
TATCAAC14150.029.2339732
CCGACCA1154.194584E-628.6003049
CATGGGT6150.028.2681274
CATGGGA11350.028.150334
ACGCAGA14400.027.7349177
CGCAGAG14500.027.5436428
TCCGACC1205.840695E-627.4086258