FastQCFastQC Report
Sun 14 Apr 2019
SRR6576742_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576742_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340898
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8360.24523464496711625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.1431513238564028No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4830.14168460947262818No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4290.1258440941278623No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4240.12437737974408766No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3830.1123503217971358No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3730.10941689302958657No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3480.10208332111071347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10300.042.027031
CCGCGAT450.001011100141.79339
GTACATG21900.038.4580271
CATGGGT6000.038.375074
ACATGGG22100.037.4218063
ATGGGTA3700.036.8299035
TACATGG22700.036.6450582
AACGCAG13000.032.169996
TCAACGC13400.031.5603684
TTAGCAC753.27488E-431.3265863
ATCAACG13500.031.3265863
CAACGCA13500.030.9785125
GGTACCT3400.030.4230658
GGGAGTC1401.8866558E-830.2077797
GGGTACC3500.029.5364977
TATCAAC15250.028.6603262
CATGGGA11150.028.6575014
ACGCAGA14800.028.2574257
CGCAGAG15050.027.8043488
CTACTAG700.00877997426.8868581