FastQCFastQC Report
Sun 14 Apr 2019
SRR6576744_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576744_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117771
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2380.20208710123884488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.16557556614107038No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1350.1146292380976641No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1330.11293102716288392No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1310.1112328162281037No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1190.10104355061942244No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1180.10019444515203234No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1180.10019444515203234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG200.002155745970.519761
TACTAGG301.3670548E-462.6576042
GGTACCT1250.048.9144948
ATGGGTA1350.048.7336925
CATACTG405.659891E-446.9932025
GTACATG10350.044.9691281
CATGGGT2550.044.22894
ACATGGG10950.043.345333
TACATGG10800.043.0771032
ATTGCAA450.001010965341.7717366
GGGTACC1600.038.1819767
TAATTGC500.001697033537.5945634
GTGTAAG651.4034781E-436.1639821
CATGGGA7250.035.0018354
ATGGGAG2850.034.626575
GTACCTG1651.8189894E-1234.2059369
AATTGCA550.002709078134.1768725
ACTGCGA1850.033.03628594
CATGGGG2900.032.4091034
GTATCAA5250.032.2376061