FastQCFastQC Report
Sun 14 Apr 2019
SRR6576745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118062
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3010.25495078856871817No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1800.15246226558926665No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1640.13891006420355406No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1630.13806305161694704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.13467500127051887No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1520.12874591316426962No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1380.1168877369517711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCC405.655926E-447.05
GTACATG9300.039.9247321
TACATGG9300.039.9247322
ACATGGG9400.039.03
ATGGGAG1900.037.1052675
CATGGGG2800.036.9285744
GTATCAA5150.036.5048561
AACGCAG5450.034.4954156
ATGGGGC851.7415405E-533.176475
CAACGCA6050.031.8512385
ATCAACG5950.031.5966383
ATGGGAT1351.3058525E-831.3333345
GCGGTAT600.004146013431.3333341
TGTAGCT600.004146013431.3333344
CCTATTC600.004146013431.3333343
TCAACGC6000.031.3333344
ACGCAGA6100.030.8196747
CATGGGA5350.030.7476624
TGGGAGA1102.9388175E-629.909096
CGCAGAG6450.029.1472878
Produced by FastQC (version 0.11.2)