FastQCFastQC Report
Sun 14 Apr 2019
SRR6576745_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576745_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118062
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2800.23716352424997034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.17363758025444256No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1640.13891006420355406No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1530.12959292575087666No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1430.12112279988480629No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1370.11604072436516408No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1360.11519371177855704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1300.1101116362589148No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1240.10502956073927258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC250.00517326156.5403821
GACGGTC250.005225653356.3966987
ATGGGCA851.4006218E-1049.761795
CATGGGC1253.6379788E-1241.357584
TACATGG7900.039.8584252
GTACATG8050.039.8006821
ACATGGG8650.036.4024933
GTAGATG651.4063074E-436.1517263
GTATCAA4750.034.7177771
ATGGGAG1502.7284841E-1134.4646455
CATGGGA5250.033.121874
AACGCAG5150.032.8524446
ACTGCTA300.004155541331.33149724-25
ATAGGAC753.2590274E-431.3314973
ACGCAGA5400.031.3314977
GGTGGTA600.004147202731.3314979
AAGTGAG600.004147202731.3314975
TGCAAGG600.004147202731.3314972
CAACGCA5550.030.4847035
ATCAACG5400.030.4611783
Produced by FastQC (version 0.11.2)