FastQCFastQC Report
Sun 14 Apr 2019
SRR6576746_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576746_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106283
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2630.24745255591204612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.16747739525606165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1700.15995032131196898No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1680.1580685528259458No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1650.15524590009691108No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1470.1383099837227026No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1280.12043318310548254No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1250.11761053037644778No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1140.10726080370332039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAT250.005225756.3926627
ATGGGAT1250.048.873645
CATGGGT1750.045.65124
GTACATG8900.044.395561
TACATGG8800.044.3237722
ACATGGG9000.043.3388023
GTATCAA4800.042.1383481
TACTAAA608.776841E-539.161572
CATGGGC1700.038.7008444
GCCTAGT500.001696169337.5951082
TGGGATG757.3866977E-637.5951086
ATGGGTA959.3533527E-734.627075
AACGCAG6200.033.35056
CATGGGA5000.032.8957184
ATCAACG6350.031.822633
CACTCTA1201.6168815E-731.3587689
AACCTCG1052.0292337E-631.3587679
ATGGCGG300.004156070331.32925640-41
GTATGGT600.004146804631.3292563
CTAGATA1052.0442221E-631.3292543