FastQCFastQC Report
Sun 14 Apr 2019
SRR6576748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250333
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7980.31877539117894965No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5380.21491373490510637No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4730.1889483208366456No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4570.18255683429671676No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4510.18016002684424348No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3860.15419461277578267No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3780.15099886950581826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.1482025941445994No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3500.1398137680609428No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3240.1294276024335585No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3160.12623185916359408No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3090.12343558380237524No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3050.12183771216739303No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2930.11704409726244643No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2870.11464728980997313No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2860.11424782190122756No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2850.11384835399248201No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2830.11304941817499091No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2810.11225048235749982No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2800.11185101444875427No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2630.10506006000007989No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2520.10066591300387884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGG405.6697655E-446.9973075
CATGGGT3100.037.901054
ATGGGTA2700.036.553465
GTATCAA8150.036.3292051
ACTATAT702.1808763E-433.56953
GTACATG14500.032.7360531
TACATGG15000.032.271482
ACATGGG14900.032.172653
TAGAGTG1052.0577663E-631.3315355
GGGTACC2800.030.2125517
GGTACCT2850.029.6825078
AACGCAG9800.029.2534246
GATAAGG650.00614682328.92141794
TACGACG650.00614682328.9214175
GCCTATC650.00614682328.9214171
CATAATG650.00614682328.9214172
ATGGGAG2900.027.550145
TGGGTAC3100.027.2887576
TCAACGC10650.026.9186424
CGACGGT700.0088255326.85567
Produced by FastQC (version 0.11.2)