FastQCFastQC Report
Sun 14 Apr 2019
SRR6576749_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576749_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230944
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7160.31003186919772757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.1987494803935153No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4540.19658445337397812No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4360.18879035610364417No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4190.1814292642372177No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4190.1814292642372177No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3730.16151101565747542No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3720.16107801025356797No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3710.16064500484966054No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3460.1498198697519745No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3220.13942774005819591No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3130.13553069142302898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2950.12773659415269503No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2850.12340654011362062No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2840.12297353470971317No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2630.11388042122765692No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2600.1125814050159346No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2580.11171539420811973No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG2510.10868435638076765No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2400.10392129693778578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT4000.042.29244
GTACATG14000.041.003061
TACATGG14250.040.561132
ACATGGG14900.038.7916873
GTATTCA500.001689882537.642151
ATGGGTA2500.037.5932435
GGGTACC2150.034.970467
GTATCAA7300.034.80611
GTATAAC550.002697668334.2201351
CATGGGG5900.032.655154
AGCGGAT600.00414021331.3548694
GCCGTGT600.004144608531.3480688
TTAGACT600.004157816531.3277024
CCGAACG300.004162086631.32770284-85
GGTACCT2450.030.708318
TGGGTAC2450.030.6883626
AACGCAG8500.029.8534565
TATACAG953.744034E-529.6788775
ATGGGGA2850.029.6788755
GTGGTCG650.006123534428.9429594
Produced by FastQC (version 0.11.2)