SRR6576750_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR6576750_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102538
Sequences flagged as poor quality	0
Sequence length	100
%GC	50

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	394	0.38424779106282553	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	286	0.2789209853907819	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	231	0.22528233435409312	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA	226	0.22040609335075775	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	213	0.20772786674208588	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG	200	0.19504964013341394	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG	197	0.19212389553141276	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT	187	0.18237141352474204	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG	186	0.18139616532407496	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	179	0.1745694279194055	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	169	0.1648169459127348	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC	167	0.16286644951140067	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG	151	0.14726247830072753	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG	151	0.14726247830072753	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT	146	0.1423862372973922	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	145	0.14141098909672511	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC	141	0.13750999629405683	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC	137	0.13360900349138857	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG	135	0.13165850709005442	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG	133	0.1297080106887203	No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC	132	0.1287327624880532	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG	128	0.12483176968538492	No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG	127	0.12385652148471785	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG	127	0.12385652148471785	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG	122	0.11898028048138251	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT	120	0.11702978408004838	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT	119	0.1160545358793813	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	114	0.11117829487604594	No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC	113	0.11020304667537888	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG	111	0.10825255027404473	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC	111	0.10825255027404473	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.10727730207337768	No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG	106	0.1033763092707094	No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG	104	0.10142581286937526	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	55	1.2712917E-8	59.815388	5
TTACACC	40	5.6537165E-4	46.997803	4
GTACATG	715	0.0	44.234104	1
ATGGGTT	55	5.2508072E-5	42.725277	5
TACATGG	760	0.0	42.050667	2
TACACCG	45	0.0010098664	41.775826	5
ACATGGG	755	0.0	41.084175	3
CATGGGT	255	0.0	36.861023	4
ACTACAC	65	1.4049161E-4	36.152157	3
GTATCAA	455	0.0	35.2741	1
GTACAGG	55	0.002648342	34.33094	1
TGGGACA	55	0.0027061524	34.18022	6
CATGGGA	325	0.0	33.259987	4
AACGCAG	510	0.0	33.174923	6
TCAACGC	530	0.0	31.923037	4
ATCAACG	545	0.0	31.906767	3
CAACGCA	535	0.0	31.624693	5
GTCTTAG	60	0.004056055	31.47003	1
AATCCAC	30	0.0041540344	31.331867	10-11
TCTTAGG	60	0.0041444344	31.331867	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content