FastQCFastQC Report
Sun 14 Apr 2019
SRR6576752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263068
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6050.22997856067632702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.15889427828546232No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3720.1414083050770143No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3330.12658324083506925No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2990.11365882585491202No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2840.10795687806954857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.049.5184251
AACGCAG11000.041.445126
TACATGG18500.040.9179272
GTACATG19150.040.5111581
ATCAACG11200.040.285393
TCAACGC11200.040.285394
CAACGCA11650.038.7292985
ACATGGG18900.038.5446623
ACGCAGA12500.036.0957077
TATCAAC13000.035.443962
CGCAGAG12750.035.387958
GGTACAC702.1805466E-433.5711563
CATGGGG7400.032.3916324
GCAGAGT14350.031.7697779
CATGGGA10000.030.5497534
CATGGGT4000.029.3747634
TAAGGTA650.006145904328.9228449
ATGGGAT2950.028.6777325
TGGGTAC2053.6379788E-1127.511976
ATGGGAG5300.027.4903435
Produced by FastQC (version 0.11.2)