FastQCFastQC Report
Sun 14 Apr 2019
SRR6576752_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576752_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263068
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5410.20565025012544286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.14634999315766267No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3470.13190505876807518No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3160.12012103334499065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2850.10833700792190612No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2780.10567609895540317No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2730.10377544969361534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7250.044.8231241
TACATGG17000.038.704472
GTACATG17450.038.3251571
ACATGGG17600.037.3850023
AACGCAG9450.036.3055576
GTACTAA651.3935602E-436.228281
TCAACGC9100.035.6360664
ATCAACG9250.035.5662733
CAACGCA9450.035.3108865
ATGGGAT2600.034.3449025
CATGGGA9000.031.8543954
GTGTTAC753.2295295E-431.3978421
ACGCAGA11150.030.3486717
ATGGGAG4050.030.1717415
CATGGGG6750.029.9396514
CGCAGAG11700.028.9220248
TATCAAC11350.028.5716462
GGTACCT2203.6379788E-1227.7717158
ATGGGGG3050.027.7366965
ATTAACC856.8034726E-427.6460533
Produced by FastQC (version 0.11.2)