FastQCFastQC Report
Sun 14 Apr 2019
SRR6576753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246827
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5710.2313361180097801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.15962597284737895No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3240.13126602843287No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2970.12032719273013082No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2950.11951690860400199No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2490.10088037370303898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT255.5363867E-575.239261
GTATCAA8950.041.507691
GTACATG18500.036.8570671
TACATGG18750.036.114842
AACGCAG10300.035.5819556
ACATGGG18850.035.1463433
ATCAACG10850.033.7782633
ATGGGTA3250.033.251935
TCAACGC11100.033.017494
CATGGGT4300.032.7812274
CAACGCA11300.032.433115
ACGCAGA11400.032.148617
CGCAGAG11400.032.148618
GTCTATG600.00414410831.3496881
GTAATAG600.00414410831.3496881
CATGGGA9450.031.3242864
CGCCGGT753.2736917E-431.3242847
TATCAAC12500.029.343312
GGTACCT2900.029.163998
ATAGACT650.00615361328.9147244
Produced by FastQC (version 0.11.2)