FastQCFastQC Report
Sun 14 Apr 2019
SRR6576753_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576753_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246827
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4600.18636534900963023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.18190878631592167No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3500.14179972207254474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3050.12356832923464613No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2840.11506034591029346No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2590.1049317943336831No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2540.10290608401836104No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2490.10088037370303898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACG200.002162791370.4849554
TACATGG17150.046.8719672
GTACATG17500.046.3534661
GTATCAA7900.044.774011
ACATGGG17900.043.8398063
ATGGGAG4650.040.4214825
CATGGGT4050.039.448374
CATGGGA10600.037.6806374
ATGGGTA2350.035.992325
AACGCAG10050.035.0671466
ATCAACG9950.034.9473153
CAACGCA10100.034.4282955
TCAACGC10250.033.924474
CATGGGG5750.033.5058944
ACGCAGA11300.031.1880347
CGCAGAG11400.030.9332548
GGTACCT2051.8189894E-1229.816648
GTTTAAT804.6749736E-429.4762231
TATCAAC11950.029.1101882
GGGTACC2101.8189894E-1229.0890317