FastQCFastQC Report
Sun 14 Apr 2019
SRR6576754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291604
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5600.19204126143674297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.14025870701362123No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3930.1347718138297143No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3170.10870907120615629No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3060.10493683214222026No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2950.10116459307828425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8500.053.0804181
AACGCAG10400.042.9311266
CAACGCA10400.042.4792185
ATCAACG10750.041.5333673
GTACATG19500.040.9728661
TCAACGC10850.040.7174074
TACATGG19950.040.0486642
ACATGGG20200.039.320353
CGCAGAG12450.036.6171388
ACGCAGA12200.036.5970237
CATGGGT4100.035.535294
TATCAAC12850.034.7458152
GTCTTAT851.7507517E-533.1752621
CATGGGA11300.032.4412964
GGTATCA4100.030.950091
GCAGAGT14700.030.6927599
ATGGGAT3700.029.2151495
CATGGGG6900.028.6076554
TATAGCC1005.31143E-528.198972
GTCCTAT1057.4047595E-526.8561631
Produced by FastQC (version 0.11.2)