FastQCFastQC Report
Sun 14 Apr 2019
SRR6576755_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576755_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268678
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5170.1924236446601508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.18125786257155407No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3600.1339893850631611No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3350.12468456665599714No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3240.12059044655684499No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3100.11537974824883318No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2720.10123642426994395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG301.3702782E-462.657235
GTATCAA9000.048.6589741
TACATGG19200.037.4474872
GTACATG19600.036.998761
ATGGGAC1700.035.935775
ACATGGG19800.035.8380433
AACGCAG11950.034.9989176
AACCGCA550.002715323834.1766747
CAACGCA12800.032.674775
ATCAACG12900.032.421483
TCAACGC13050.032.4089134
CATGGGT4950.031.3286194
CATGGGA11200.031.0488974
GACGGCT552.9680468E-629.90458924-25
TATCAAC14300.029.5759682
GCGGTAT650.006089119728.9780521
ACGCAGA14450.028.9437437
CATGGGG5700.028.8553054
CGCAGAG14900.028.0852818
ATGGGTA3050.027.733535
Produced by FastQC (version 0.11.2)