FastQCFastQC Report
Sun 14 Apr 2019
SRR6576756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342940
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8490.24756517175016038No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5480.15979471627689976No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4580.13355105849419724No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4170.12159561439318832No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3820.1113897474776929No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3610.10526622732839565No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3540.10322505394529656No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3470.10118388056219746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6550.050.9441151
GTACATG20900.040.926261
TACATGG21150.040.886922
ACATGGG22100.038.2787063
ATTAGGG500.00170076437.5981942
GTATAGG906.1757237E-736.5537951
TCAACGC9400.034.4983374
ATCAACG9500.033.6404883
AACGCAG9800.033.5698136
TAAGGTA702.1824351E-433.5698134
ATAAGAC851.7517856E-533.1748773
CAACGCA9800.033.0902445
TACACTA1001.4078687E-632.898425
CATGGGT6550.031.5710014
TAATGGT1351.3220415E-831.331834
ATTGACT600.00415792831.3318278
CATGGGG7500.030.7051894
CATGGGA10700.029.8677224
ACGCAGA10950.029.6150137
CGCAGAG11300.028.6977358