FastQCFastQC Report
Sun 14 Apr 2019
SRR6576757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316484
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8590.27141972421986577No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5520.17441640019716637No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4310.13618381971916432No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4100.1295484131899243No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3820.12070120448427094No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3780.11943731752632045No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3710.11722551534990709No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3410.10774636316527851No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3200.10111095663603846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.047.254741
CATGGGT5700.039.5685734
GTACATG19100.036.1690061
TACATGG19600.035.4860952
ATCAACG11250.035.084133
ATGGGTA2950.035.0416535
TCAACGC11500.034.321434
ACATGGG20350.034.1728553
AACGCAG11700.034.1363456
CAACGCA11700.033.734745
GTATAAC600.004158588631.3300691
GCAAACG753.27222E-431.3300671
GGTACCT3150.031.3251198
GTTAGAC600.00416180731.3251173
GGGTACC3050.030.811597
GGTATCA4150.030.5751271
ACGCAGA12900.030.2323827
TATCAAC13450.029.35012
CGCAGAG13300.029.3231358
GCGCTCT650.006155402428.91549594