Basic Statistics
Measure | Value |
---|---|
Filename | SRR6576758_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 149407 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG | 380 | 0.25433881946629006 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 373 | 0.2496536306866479 | No Hit |
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA | 208 | 0.1392170380236535 | No Hit |
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA | 201 | 0.13453184924401132 | No Hit |
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT | 190 | 0.12716940973314503 | No Hit |
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA | 185 | 0.12382284631911489 | No Hit |
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA | 177 | 0.11846834485666669 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 167 | 0.11177521802860642 | No Hit |
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA | 167 | 0.11177521802860642 | No Hit |
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA | 158 | 0.10575140388335218 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTATGCC | 25 | 0.005230543 | 56.390762 | 4 |
GTATCAA | 530 | 0.0 | 45.38609 | 1 |
GTATATG | 55 | 5.1526054E-5 | 42.878128 | 1 |
AATCCGT | 25 | 0.0017013807 | 37.59384 | 70-71 |
ATTATGG | 50 | 0.0016986585 | 37.59384 | 3 |
TATCCTG | 100 | 3.2985554E-8 | 37.59384 | 5 |
ATCAACG | 675 | 0.0 | 36.89766 | 3 |
CAACGCA | 680 | 0.0 | 36.626354 | 5 |
TTATCCT | 105 | 5.068614E-8 | 35.803658 | 4 |
AACGCAG | 690 | 0.0 | 35.41449 | 6 |
GTACAAG | 80 | 1.1244127E-5 | 35.374454 | 1 |
TCAACGC | 705 | 0.0 | 35.327545 | 4 |
GGTATCA | 265 | 0.0 | 33.81709 | 1 |
TCTTAGG | 70 | 2.178046E-4 | 33.565933 | 2 |
GTACATG | 735 | 0.0 | 32.727386 | 1 |
TACATGG | 735 | 0.0 | 32.606903 | 2 |
ACATGGG | 765 | 0.0 | 31.942482 | 3 |
CGCAGAG | 760 | 0.0 | 31.53431 | 8 |
ATATGAT | 60 | 0.004152839 | 31.3282 | 3 |
GAGATAG | 60 | 0.004152839 | 31.3282 | 2 |