FastQCFastQC Report
Sun 14 Apr 2019
SRR6576758_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576758_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149407
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3800.25433881946629006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.2496536306866479No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2080.1392170380236535No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2010.13453184924401132No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1900.12716940973314503No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1850.12382284631911489No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1770.11846834485666669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.11177521802860642No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1670.11177521802860642No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1580.10575140388335218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCC250.00523054356.3907624
GTATCAA5300.045.386091
GTATATG555.1526054E-542.8781281
AATCCGT250.001701380737.5938470-71
ATTATGG500.001698658537.593843
TATCCTG1003.2985554E-837.593845
ATCAACG6750.036.897663
CAACGCA6800.036.6263545
TTATCCT1055.068614E-835.8036584
AACGCAG6900.035.414496
GTACAAG801.1244127E-535.3744541
TCAACGC7050.035.3275454
GGTATCA2650.033.817091
TCTTAGG702.178046E-433.5659332
GTACATG7350.032.7273861
TACATGG7350.032.6069032
ACATGGG7650.031.9424823
CGCAGAG7600.031.534318
ATATGAT600.00415283931.32823
GAGATAG600.00415283931.32822