FastQCFastQC Report
Sun 14 Apr 2019
SRR6576759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370411
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8870.2394637308287281No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5550.1498335632581106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.14065457019364977No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4970.13417528097167739No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4670.12607616944421196No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4670.12607616944421196No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4140.11176773907902303No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3950.1066383017782949No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3950.1066383017782949No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3790.10231877563031334No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3710.10015901255632258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA201.8364542E-593.997854
CCCGCTA301.3700961E-462.6652263
GTATCAA9800.046.5256231
GCAAACG555.27265E-542.732061
TGCGTAT450.001013179441.77681794
GTACATG23550.039.9195481
TACATGG24100.039.203562
ACATGGG24750.037.9789243
CGCTAAA500.001700730537.5991365
ATCAACG12950.035.2038273
AACGCAG13050.034.934066
ATGGGTA4250.034.2815635
CATGGGT6050.034.1810344
CAACGCA13500.033.7695925
TCAACGC13600.033.521294
TGGGTAC4050.032.493086
CATGGGA12650.031.5803014
GGGTACC4200.030.2135937
GGTACCT4050.030.1721468
CATGGGG8450.030.0348134