FastQCFastQC Report
Sun 14 Apr 2019
SRR6576759_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576759_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370411
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8910.2405436123657235No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6300.1700813420767742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.1646819343917972No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5310.14335427403613824No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4920.13282542905043315No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4600.12418637675447003No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4560.12310649521747465No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4290.11581729484275574No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4020.10852809446803685No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4020.10852809446803685No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3860.10420856832005529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.053.898671
ATCAACG13850.040.0458533
TCAACGC14150.039.5294
GTACATG23950.038.352491
CAACGCA14500.038.2506945
TACATGG23650.037.9601142
ATAGTAC500.001700010537.602383
CATGGGG7900.037.483384
AACGCAG14850.037.3491636
ACATGGG23800.037.3258863
ATGGGGA4750.033.6442345
ATGGGTA4100.033.2460025
TATCAAC16800.033.013992
CATGGGT6200.031.8407214
TATACAG1658.54925E-1131.3353145
TACCGTC753.272668E-431.3310857
ACGCAGA17550.031.3310837
CGCAGAG17750.030.9780588
GGGTACC4250.030.9624827
GGTACCT4400.029.9069428