FastQCFastQC Report
Sun 14 Apr 2019
SRR6576760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345453
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8330.24113265769873182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.16007966351428413No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5280.15284278903352988No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5240.1516848891166092No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4710.13634271521741018No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4180.12100054131821117No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4000.11578999169206809No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3740.10826364223208366No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3640.10536889243978197No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3640.10536889243978197No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3600.1042109925228613No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3470.10044781779286907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11250.051.386821
AACGCAG13900.041.5840346
ATCAACG14200.039.7126853
TACATGG23550.038.717742
CAACGCA14600.038.6246645
TCAACGC14700.038.361914
GTACATG23900.038.34741
ACATGGG24250.036.8195273
GGTATCA4700.036.000111
ACGCAGA16300.035.167847
CATGGGG7500.035.0883644
CGCAGAG16500.034.741568
AACCGCG550.002718205634.1720287
TGCCCGC1001.4073466E-632.90011
TATCAAC17450.032.3210142
CATGGGT6550.032.28554
GCAGAGT18950.030.4978629
TGGGTAC3400.030.4074576
CATGGGA12300.029.8006554
ATGGGTA3550.029.1226355
Produced by FastQC (version 0.11.2)