FastQCFastQC Report
Sun 14 Apr 2019
SRR6576760_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576760_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345453
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8490.24576425736641452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.1925008611880632No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5780.1673165379950384No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5150.14907961430353767No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4660.13489534032125933No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4400.1273689908612749No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4380.12679004090281457No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4130.1195531664220603No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3900.1128952418997664No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3760.10884259219054401No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3690.10681626733593283No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3670.10623731737747248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10300.049.3444821
TAACGCA450.001013107741.776624
GTACATG22200.038.5807381
TACATGG22500.037.8133162
AACGCAG13850.037.3274886
ATCAACG14300.036.4815063
ACATGGG23100.036.011993
TCAACGC14600.035.409984
CAACGCA14750.034.731245
ACGCAGA16250.031.8098967
CATGGGA10750.031.4781974
TAGCACC600.004157553431.3324624
CGCAGAG16650.031.0681828
TATCAAC16500.031.0521182
CATGGGG7600.030.301794
ACCGTGC804.7586428E-429.3912078
CATGGGT7400.027.3100554
GCAGAGT19000.027.2255389
CAGTACG700.008803926.871969
TTTAGAC700.00882881226.85643
Produced by FastQC (version 0.11.2)