FastQCFastQC Report
Sun 14 Apr 2019
SRR6576761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286973
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4680.16308154425677676No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3440.11987190432549405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.11290260756238392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8300.060.589241
CAACGCA10450.048.5732655
TCAACGC10800.046.9991264
ATCAACG10850.046.7825433
AACGCAG11100.046.1522986
TATCAAC12000.042.2992172
GTACATG18550.040.2849661
TACATGG18600.040.1766742
ACGCAGA12650.040.1257367
CGCAGAG12800.039.6555148
ACATGGG19050.038.734193
GCAGAGT14550.034.8859529
CATGGGA10950.034.766484
ATGGGAG5700.032.9818465
GTATAGG600.00415620631.3327521
AGAGCGT600.00415620631.3327526
CATGGGC3500.030.8851454
GGTATCA2750.030.7630671
CATGGGG5550.030.4859224
CATGGGT4100.028.6580054
Produced by FastQC (version 0.11.2)