FastQCFastQC Report
Sun 14 Apr 2019
SRR6576761_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576761_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286973
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5860.20420039515912647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.1825955751934851No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4610.16064229038968822No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3460.12056883400180506No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3250.11325107240053943No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3220.11220567788607291No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3140.10941795918082886No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3060.10663024047558481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7100.061.0579451
ATCAACG9800.044.6075823
GGTATCA2550.042.501121
CTAACGC450.001012063941.7829673
TCAACGC10500.041.6337434
CAACGCA10650.041.047355
AACGCAG10900.039.6746566
GTATAGG853.8632606E-738.8053741
GTACATG18400.038.157611
TACATGG18400.038.064512
ACATGGG18700.037.453853
TATCAAC12200.035.832322
CATGGGG6650.035.342744
ACGCAGA12750.033.9120257
CGCAGAG12900.033.153388
GTCTAAC1201.608314E-731.4138721
ATGGGGA3750.030.0837355
CATGGGA10650.029.1303794
GCAGAGT14500.028.8468329
CTTACAC1005.3053234E-528.20353
Produced by FastQC (version 0.11.2)