FastQCFastQC Report
Sun 14 Apr 2019
SRR6576762_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576762_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239126
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6340.2651321897242458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.19654909963784783No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3550.14845729866262974No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3510.14678454036783956No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2910.12169316594598664No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2580.10789291001396753No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2490.10412920385068959No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2410.10078368726110921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5250.057.455961
GGTATCA2050.045.982291
ATAGACG555.2550764E-542.748498
GTCCTCG450.001012997541.7722936
ATCAACG7900.039.260673
TACATGG14150.038.857092
GTACATG14100.038.7751351
CGAAATC250.001696890237.61866812-13
TCAACGC8250.037.5950624
CAACGCA8350.037.1448255
ACATGGG14750.036.3206523
AACGCAG8650.035.8565646
TATCAAC9650.032.1408582
ATGGGAG4100.032.093355
AGCTACG300.004144232331.35545368-69
GGGTACT600.004157122731.329224
CCGTATT300.004161246631.3292226-27
ACGCAGA10100.030.708847
CATGGGA8700.030.2489014
CGCAGAG10550.029.417448