FastQCFastQC Report
Sun 14 Apr 2019
SRR6576763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280812
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5690.20262666837599533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.14992236798997194No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3590.12784353944988106No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3200.11395524407788842No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2930.10434027035881657No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2900.10327193994558637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7550.052.9122051
TACATGG18650.037.548392
GTACATG18800.037.4987951
CATGGGT4600.036.7814254
ACATGGG19050.036.513263
TGGGTAC2700.034.813696
CATGGGA9550.034.4491544
GGCCTAA550.00271395834.1807171
AACGCAG11600.033.6282276
GTACTAG851.75047E-533.1754041
ATCAACG11800.032.6599663
TCAACGC11950.032.2500084
CAACGCA12200.031.5891465
CGATAAT300.00415984231.33232366-67
GGGTACC2900.030.792117
TATCAAC13150.030.0218472
ATGGGTA3400.029.0284775
GGTACCT3000.028.1990938
ATGGGAG4700.027.99915
ACGCAGA14000.027.8633887
Produced by FastQC (version 0.11.2)