FastQCFastQC Report
Sun 14 Apr 2019
SRR6576763_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576763_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280812
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.20725610016665955No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5060.18019172969816105No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3940.1403073942709001No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3330.11858467586855262No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3220.11466746435337521No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3160.1125308035269148No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3140.111818583251428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8500.049.9224551
AACGCAG10950.039.064546
ATCAACG10950.038.635263
TACATGG19100.038.3925252
GTACATG19150.038.162361
ACATGGG19300.037.994673
TCAACGC11350.037.6878174
ATGACGG500.001698730937.6049845
CAACGCA11600.036.8755765
CATGGGG6800.034.5634044
TAGACCC550.002711765234.186354
ACGCAGA12600.033.94297
CGCAGAG13000.032.8985028
CATGGGA10350.032.699994
ATGGGAG4600.031.6781145
TGTATAG1351.3178578E-831.337492
TATCAAC13650.031.3374882
ATGGGAC2550.031.3374885
CTAGTAC600.00415298131.3374883
GTACGGG300.004160112731.33190782-83
Produced by FastQC (version 0.11.2)