FastQCFastQC Report
Sun 14 Apr 2019
SRR6576764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263463
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5140.19509380823872802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.1715610920698542No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3640.13815981750758172No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3320.12601389948493716No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3240.12297741997927604No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3080.11690446096795375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8900.044.883381
CTAGTAC555.2808347E-542.715113
GTCTAAC608.8104905E-539.162951
TATTGGG500.001700439637.596432
GTACATG18050.036.4508321
TGCCCGC651.4113217E-436.1504141
TACATGG18350.036.1110152
AACGCAG10800.035.2399646
ACATGGG18450.034.8897933
CATGGGT3800.033.385234
ATCAACG11600.033.214683
TCAACGC11650.033.072134
GGGTATG1001.4067409E-632.8968771
CAACGCA11700.032.5291985
TCGGACA300.00416475531.32441118-19
CATGGGG6100.030.8108984
ACGCAGA12950.030.1149797
CGCAGAG13000.029.999158
ACTAGGC1102.9635812E-629.9005783
TATCAAC13400.029.8106042
Produced by FastQC (version 0.11.2)