FastQCFastQC Report
Sun 14 Apr 2019
SRR6576764_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576764_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263463
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5810.22052432409864006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.21710828465477125No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3760.14271453676607343No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3550.13474377806371293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3450.1309481786816365No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3120.11842270072078431No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2940.11159062183304677No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2910.11045194201842383No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2850.108174582389178No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2650.10058338362502514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG301.3702078E-462.657432
GTATCAA10850.050.317841
CATGGGT4000.038.7692874
AACGCAG13600.038.7001766
ATCAACG13750.038.619763
TCAACGC14100.037.6611144
TATAACG500.001700878337.594462
CAACGCA14450.036.748915
GTACATG19150.036.373631
TACATGG19900.035.185772
ACGCAGA15100.034.855797
ACATGGG20300.034.4924553
TACAATA550.002715187634.176782
TATCAAC15900.033.693152
CGCAGAG15550.033.570388
ATGGGTA2750.032.4679375
ATGGGTT600.004158213731.3287145
TATAGAG600.004158213731.3287146
GCAGAGT17050.030.8928019
GGTACCT2300.030.670948