FastQCFastQC Report
Sun 14 Apr 2019
SRR6576766_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576766_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325786
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10240.31431676008177145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.2587588171376302No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7010.21517192267316582No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5850.17956572719515262No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5540.17005027840361464No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5490.16851552859852786No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5350.1642182291442849No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5110.15685143007986838No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4680.1436525817561221No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4630.14211783195103533No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4390.13475103288661883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.13168153327644527No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4080.12523558409508082No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4070.12492863413406347No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3990.12247303444592464No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3810.11694793514761223No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3780.11602708526456017No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3770.11572013530354282No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3740.11479928542049075No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3690.11326453561540396No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3660.1123436857323519No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3560.10927418612217837No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3500.10743248635607425No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG3490.10712553639505688No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3360.10313518690183127No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG3290.10098653717470978No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC3280.10067958721369243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGT902.5102054E-1046.9934733
GTATCAA12600.045.2328151
TAGGTAT1151.8189894E-1244.950285
GTACATG21250.041.6713641
GTCTTAG1700.041.5605351
TACATGG21350.040.953032
TTAGGTA1051.1350494E-940.280124
ACATGGG21900.039.053943
AACGCAG14500.038.8911556
GGTACCT2800.038.6255048
TGGGTAC3150.035.8045546
GGGTACC3050.035.4377027
ATCAACG16500.034.461883
TCAACGC16550.034.357774
TAATAGA550.002716012334.177074
ATAATAG1256.2464096E-933.8353043
GGTATAG1256.2464096E-933.8353047
CAACGCA16850.033.746065
TCTTAGG2200.032.0508462
CATGGGT6900.032.0100484
Produced by FastQC (version 0.11.2)