FastQCFastQC Report
Sun 14 Apr 2019
SRR6576767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301092
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.2334834535623663No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6010.19960676470978966No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4120.13683525301236832No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3820.1268715209969046No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3430.11391866937680178No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3360.11159379857319357No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3120.1036228129608226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1036228129608226No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3060.10163006655772987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9900.054.604161
AACGCAG13150.040.387196
ATCAACG14000.037.9351123
TCAACGC14050.037.800114
CAACGCA14450.037.0789955
TACATGG19800.035.136592
GTACATG20550.034.3117261
ACATGGG20450.034.2439543
TATCAAC16000.033.7863242
TGCCCGC702.1792644E-433.5764731
GGGGTAT702.181392E-433.5708967
ACGCAGA15900.033.4019857
CGCAGAG15950.033.2972768
ATGGGAG4450.032.7410555
ATATACT1151.1327393E-732.6951334
CATGGGA9700.032.46344
TATACTC902.5879004E-531.3328345
GCAGAGT17550.030.797239
GCAAACG650.00614254328.9274221
AATAGGC650.00614753128.9226173