FastQCFastQC Report
Sun 14 Apr 2019
SRR6576767_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576767_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301092
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.27367050602473664No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5620.18665391308968687No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4890.1624088318520585No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4130.13716737741288376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.13318188460669828No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3770.12521089899432733No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3320.11026530097113175No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3090.10262643975927624No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3050.10129794215721441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.053.5156061
TATCACG450.00101319441.774162
ATCAACG14700.040.2822273
AACGCAG15000.039.7898866
CAACGCA15050.039.345435
TCAACGC15150.039.0857244
GGTATCA4450.037.08011
TATCAAC16900.035.594552
TACATGG20100.035.30542
GTACATG20200.035.0084421
ACGCAGA17300.034.4999057
CATGGGA8450.034.4822244
AGTGATA550.002714990934.178868
ACATGGG21100.033.632163
CGCAGAG17800.033.0027588
GTATCAC600.004094068431.4298021
GCAGAGT19500.030.3666029
GTATGGA804.6874367E-429.4654391
CATGGGG8300.029.4432354
GTATATA1154.0839295E-628.6967741