FastQCFastQC Report
Sun 14 Apr 2019
SRR6576768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286079
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.2369974727260652No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6310.22056844438074796No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4220.1475117013132736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.1415692868053929No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3920.13702508747583708No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3760.13143222676253763No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3310.11570230600638286No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3290.11500319841722041No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3000.10486613837436512No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2890.10102104663397174No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2890.10102104663397174No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2880.1006714928393905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.054.47961
CCGAACT601.6004069E-647.0020071
AACGCAG17750.039.4414146
ATAACGC608.8234025E-539.1546483
ATCAACG18300.038.7695243
TCAACGC18600.038.1442074
CAACGCA18650.038.0419435
CGCAGAG19250.036.3680578
ACGCAGA19350.036.180117
GTACATG19100.034.9439051
TATCAAC20750.034.2038732
GCAGAGT20600.033.9847159
TACATGG19300.033.851192
ACATGGG19950.032.9723363
TAGACTG600.00416205331.3237175
TAACGCC600.00416205331.3237174
AACGCCG804.780594E-429.3659885
AGAGTAC19650.029.29126510-11
CATGGGC3900.028.9142024
CATGGGA9400.027.9914114
Produced by FastQC (version 0.11.2)