FastQCFastQC Report
Sun 14 Apr 2019
SRR6576768_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576768_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286079
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.31879306065806995No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5470.19120592563592576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.15275500823199187No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4320.15100723925908577No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3970.13877285644874318No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3400.1188482901576138No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3330.11640141359554529No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3310.11570230600638286No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3220.1125563218551519No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3120.10906078390933974No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2920.10206970801771538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.10102104663397174No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2870.1003219390448093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11800.046.6920931
ATAAGGT555.2781477E-542.720363
TACGCTC450.001010000841.80024794
AACGCAG14800.037.7844286
TACATGG19450.036.240922
GTACATG19300.036.1113661
ATCAACG15600.035.2442973
ACATGGG19850.034.8004153
CAACGCA16100.034.441635
GCCTATA550.00268794334.2481231
AGCGTGT550.002706467434.20028
TCAACGC16150.034.044034
GTGCTAA1001.3851168E-632.9638181
ATGGGTA2450.032.6069685
ACGCAGA17550.031.8637927
GTATAAG753.2324815E-431.3941151
GAGGGTC600.00414488231.3501829
GACGAAC300.004162546231.32826252-53
CGCAGAG17800.031.1740578
CATGGGG7200.030.6755924
Produced by FastQC (version 0.11.2)