FastQCFastQC Report
Sun 14 Apr 2019
SRR6576769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137837
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3200.23215827390323354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.14509892118952095No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1990.14437342658357336No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1970.14292243737167815No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1630.11825562076945959No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1580.11462814773972156No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1550.11245166392187873No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1460.10592221246835029No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1420.10302023404455989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA200.00215906370.498915
GCAAACG301.3621025E-462.711211
TGTTCTA250.005211611756.4400832
CCGCTAA250.005226614456.3991244
GTATCAA5750.043.3525281
TTAGGGC555.2593103E-542.7266163
TATTAGG450.001007318141.807472
AACGCAG6950.037.193676
TAGGGCT651.4071733E-436.153294
CAACGCA7150.035.4959535
TCAACGC7200.035.2494544
ATCAACG7300.034.7665863
GGCTATT550.002708961334.181297
ACGCAGA7600.034.0126347
CGCAGAG7750.033.3543248
TATCAAC7700.032.3736462
GATCACG300.00415585931.33285122-23
GAATAAC600.004148714731.3328519
GGTACCT902.5777954E-531.332858
ACATGGG9500.031.1679423